Chlamydomonas transformation

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August undefined, 2024

WebMay 16, 2024 · Chlamydomonas reinhardtii cells were transformed by four different approaches for each promoter sequence: either expression cassettes for luciferase and selectable marker were delivered traditionally on two separate plasmids (pProm-Chlamy and pUC-Arg7-lox-B) or as a tandem assembly in pFusion plasmids, both in a supercoiled … WebThe objective of this study was to determine the efficacy of three commonly used Agrobacterium strains in genetic transformation of the microalga Chlamydomonas reinhardtii. Three different strains of Agrobacterium tumefaciens (EHA 101, EHA 105, LBA

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WebDec 4, 2012 · The transformation rate observed in Chlamydomonas reinhardtii transformed with the linearized pSI103 control is 1.8 × 10 −6 transformants per cell and µg of DNA while the transformation with the promoter-less cassette was 2.6 × 10 −7, which means about 14% of the transformation rate observed for the control plasmid .WebAbstract: Mitochondrial transformation of Chlamydomonas reinhardtii has been optimized by using a particle-gun device and cloned mitochondrial DNA or PCR fragments. A respiratory-deficient strain lacking a 1.2-kb mitochondrial DNA region including the left telomere and part of the cob gene could be rescued as well as a double-frameshift …philipp hahn built the first scale to work

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WebChlamydomonas reinhardtii C. reinhardtii ), a unicellular green alga, is an ideal model eukaryotic 30 organism in many biological processes, including organelle biogenesis, biodiesel metabolism,...WebOct 4, 2024 · Chlamydomonas reinhardtii (Chlamydomonas) is a unicellular green alga that has been used as a model organism for more than 60 years to study diverse processes, such as photosynthesis, chloroplast biogenesis, flagella, cell cycle control and sexual reproduction (Harris, 2001, 2008 ). WebFeb 7, 2024 · Transformation into Chlamydomonas reinhardtii Cultures of wild-type (WT) C. reinhardtii strain CC125 (obtained from chlamycollection.org) were grown and transformed via electroporation using modified methods of Rasala et al. ( 2012 ). Cells were grown under 100 μmol m −2 s −1 light and maintained at 25 °C on a shaker table at 125 … philipp handschuh

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Chlamydomonas Facts, Structure, Life Cycle, & Classification

WebJun 13, 2016 · For the latter two genes, we employed co-transformation of vectors that confer resistance to hygromycin due to low delivery efficiency in Chlamydomonas. Interestingly, the vectors used in our ... WebApr 1, 1998 · CHLAMYDOMONAS reinhardtii is a unicellular, eukaryotic green alga that has been used to elucidate aspects of photosynthesis, phototaxis, flagella assembly, cell wall biogenesis, gametogenesis, cell cycle events, mating processes, and nuclear/chloroplast interactions (for review, see Rochaix 1995).There are many advantages of using C. …philipp hammerich hamburgWebChlamydomonas reinhardtii is a model green microalga capable of heterotrophic growth on acetic acid but not fatty acids, despite containing a full complement of genes for β-oxidation. Recent reports indicate that the alga preferentially sequesters, rather than breaks down, lipid acyl chains as a means to rebuild its membranes rapidly. Here, we assemble … trulieve fernandina beach fl

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Chlamydomonas transformation

Chloroplast Transformation in Chlamydomonas with High Velocity ...

WebIn another study, a Chlamydomonas sp. was investigated for its ability to transform lindane, naphthalene and phenol [9]. The halogenation of 4-chloro-3,5-dinitrobenzoic acid by a Chlamydomonas sp. gave 2-hydroxymuconic semialdehyde [10]. Liebe and Fock [11] showed that Chlamydomonas reinhardtii was able to remove some of the toxic iso-octane- WebMar 1, 2024 · Experienced researcher in the fields of genetics and molecular biology focusing on the sexual development of the green alga …

Chlamydomonas transformation

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WebMar 1, 2004 · Co-cultivation of Chlamydomonas with Agrobacterium resulted in the generation of hygromycin resistant colonies indicating the transfer of T-DNA into algal … WebJan 11, 2024 · To integrate exogenous DNA fragment into the nuclear genome, various techniques have been developed for transformations of Chlamydomonas cells. The old and successful biolistic technology could deliver exogenous DNA into the genome [ 13 ]. However, this method needs a special DNA delivery system and yields few transformants.
WebFeb 1, 2024 · Nuclear transformation method of C. reinhardtii. The glass bead method is a Chlamydomonas nuclear transformation technology proposed by Kindle et al., in 1990. The exogenous DNA, cell wall-free Chlamydomonas and glass beads are thoroughly mixed and then vortexed. WebFeb 1, 1990 · By using a method in which cell-wall-deficient Chlamydomonas reinhardtii cells were agitated in the presence of DNA, glass beads, and polyethylene glycol, …
WebMar 14, 2006 · We present here an optimized biolistic transformation procedure of Chlamydomonas mitochondria using cloned mitochondrial DNA or PCR fragments. In contrast to previous reports, both a deletion and a frameshift mutant could be rescued, and high transformation efficiency has been achieved (100–250 transformants per …WebApr 13, 2024 · Benzophenones (BPs) are one of the most widely used UV-filters and previous flow cytometric studies have shown that these aquatic emerging pollutants alter the physiology of the freshwater microalga Chlamydomonas reinhardtii. In order to obtain a more detailed study of the different cellular metabolic pathways affected, changes …
WebAbstract. Bombardment of three mutants of the chloroplast atpB gene of Chlamydomonas reinhardtii with high-velocity tungsten microprojectiles that were coated with cloned …}

WebChlamydomonas is an excellent model system to study the regulation of cilia and flagella. All major structural components of cilia are conserved in this unicellular green alga. … philipp hannuschWebFeb 1, 2024 · Chlamydomonas reinhardtii is a model organism with three sequenced genomes capable of genetic transformation.C. reinhardtii has the advantages of being …trulieve first time discount codeWebChlamydomonas, genus of biflagellated single-celled green algae (family Chlamydomonadaceae) found in soil, ponds, and ditches. Chlamydomonas species can … trulieve flower strainsWebChlamydomonas reinhardii with yeast DNA ... transformation was the plasmid p Yearg4 containing a yeast replication origin and the yeast arg4 locus3•4 , which is analogousphilipp hahn speakerWebThis proportion of cotransformants is within the range normally obtained using conventional transformation methods with Chlamydomonas (Kindle et al. 1989; Tam and Lefebvre 1993). Clone 104 cells and three other non-HSV–GSP1-expressing clones originally in the culture wells adjacent to the HSV-positive clones were used as controls. trulieve flower philipp hanebuthWebMay 24, 2024 · Hello, I Really need some help. Posted about my SAB listing a few weeks ago about not showing up in search only when you entered the exact name. I pretty … philipp hanich