WebThe PicoHarp 300 is a high-end, easy to use, plug and play Time-Correlated Single Photon Counting (TCSPC) system. It is connected to a PC through a USB 2.0 interface. The … WebFLIMfit is a software tool and an OMERO client that is designed to facilitate analysis and visualisation of time-resolved data from FLIM (Fluorescence Lifetime Imaging) measurements including time-correlated single photon counting (TCSPC) and wide-field time-gated imaging. It can incorporate image segmentation and utilises a highly efficient ...
Frontiers A Review of New High-Throughput Methods Designed …
WebIn time-correlated single photon counting experiments like TCSPC-FLIM, the achievable time resolution is typically limited by the laser pulse duration and the electronic jitter of the detector and TDC. With the availability of ultrafast lasers, the pulse duration is not an issue for most experiments, while the detector's electronic jitter is ... Web所有SPC模块都使用多维TCSPC计数，因此，SPC模块可用于经典的荧光寿命应用以及荧光寿命成像（FLIM），多波长FLIM，同时荧光和磷光寿命成像（FLIM / PLIM），快速时间序列FLIM，荧光相关光谱（FCS），单分子实验，反聚束实验和其他多维光子记录任务。 所有TCSPC模块可兼容各类探测器，如 PMT, SPAD, InGaAs-SPAD 及 超导纳米线单光子探 … on the square regalia
Fluorescence lifetime imaging microscopy (FLIM)
WebOptically-sectioned FLIM is most commonly implemented on confocal and two photon excitation (TPE) microscopes using time-correlated single photon counting (TCSPC), which is a photon-efficient method of measuring fluorescence lifetime . Single point scanning confocal and TPE microscopes are limited by photobleaching or phototoxicity within the ... WebThe phasor plot, also known as the AB plot or the polar plot (2-6), is a graphic representation of the raw FLIM data. Transforming FD or TCSPC FLIM data into the phasor plot is described below. 3.1 The phasor plot for FD FLIM data For one modulation frequency (ω), the FD FLIM data measurements at each pixel location are composed of both the WebOptically-sectioned FLIM is most commonly implemented on confocal and two photon excitation (TPE) microscopes using time-correlated single photon counting (TCSPC), … on the square gun shop springtown tx