Greengate cloning
Webgreen gate cloning 중인데 cloning이 처음입니다 mod c인 cds를 만들던 중 프라이머 Forward에... WebGreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors. All these make GreenGate versatile and applicable cloning system …
Greengate cloning
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WebNational Center for Biotechnology Information Webdestination vector for GreenGate cloning method, contains 2x a set of modules from A-F, "Driver line", tissues-specific promoter, Dex-inducible mTurquoise2 expression:
WebCloning is performed by pipetting in a single tube all plasmid donors, the recipient vector, a type IIS restriction enzyme and ligase, and incubating the mix in a thermal cycler. …
WebJan 4, 2024 · Understanding the context-specific role of gene function is a key objective of modern biology. To this end, we generated a resource for inducible cell-type specific trans-activation based on the well-established combination of the chimeric GR-LhG4 transcription factor and the synthetic pOp promoter. Harnessing the flexibility of the GreenGate … WebGreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J PLoS One. 2013 Dec 20;8(12):e83043. doi: 10.1371/journal.pone.0083043. PubMed Plasmids from Article ...
WebThe characteristic of GreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors fused with different tags. On the basis of the abundant six modules provided by GreenGate toolkit, it is also possible to use empty modules to carry out modifications for specific research. The availability of ...
WebDec 20, 2013 · GreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of … shutter drawingWebFor this study, plasmid constructs were generated via GreenGate cloning . The protein-coding regions of MsSND1, ... For ectopic expression of transcription factors, the respective GreenGate construct was transformed into Agrobacterium tumefaciens ASE (pSOUP +). Selected transgenic clones were incubated in liquid LB medium containing the ... shutter double vanity no topWebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were … shutter door slim bathroom cabinetWebDec 20, 2013 · GreenGate cloning is simple and efficient since it uses only one type IIS restriction endonuclease, depends on only six types of insert modules (plant promoter, N-terminal tag, coding sequence, C-terminal tag, plant terminator and plant resistance cassette), but at the same time allows assembling several expression cassettes in one … the painted marlin grille south padre islandWebApr 27, 2024 · An important prerequisite for a molecular toolkit is a universal and cost-effective cloning method that can be easily adopted by different laboratories. To this end, we generated a set of plasmids based on the GreenGate cloning system (Lampropoulos et al., 2013; Figure 1; Supplemental Table S1). This Golden Gate cloning system allows … shutter dresses for womenWebThis plasmid is designed for Golden Gate cloning using the BsaI enzyme. Plasmid Features (listed as bp in full plasmid sequence): BsaI site #1 = 2241-2251bp D-dummy = 2252-2297bp ... GreenGate - A Novel, Versatile, and Efficient Cloning System for Plant Transgenesis. Lampropoulos A, Sutikovic Z, Wenzl C, Maegele I, Lohmann JU, Forner J ... shutter downloaderWebThe characteristic of GreenGate cloning technology is sufficient to simultaneously construct a variety of effector expression vectors fused with different tags. On the basis of the … the painted lady tattoo studio